Biobench Solutions

Diagnostics Services

Biobench Solutions offer wide portfolio of diagnostic laboratory services, including a comprehensive range of routine and advanced tests, across all major disease areas.

Molecular Diagnostics Cytogenetics FISH Invitro Fertilisation

Molecular Diagnostics

We use different techniques to detect the presence of genetic material or proteins associated with disease, and facilitate the practice of personalized medicine. Test range includes infectious disease, oncology, immunology, coagulation and pharmacogenomics.

Cytogenetics

This evolving technology is vital in unrevealing genetic etiology behind various diseases. Our scientists work with clinicians to understand the link between genetic aberration and disease pathophysiology.

Stem cells are undifferentiated, or “blank,” cells. This means they’re capable of developing into cells that serve numerous functions in different parts of the body. Most cells in the body are differentiated cells. These cells can only serve a specific purpose in a particular organ. For example, red blood cells are specifically designed to carry oxygen through the blood

Stem cells are cells that haven’t differentiated yet. They have the ability to divide and make an indefinite number of copies of themselves. Other cells in the body can only replicate a limited number of times before they begin to break down. When a stem cell divides, it can either remain a stem cell or turn into a differentiated cell, such as a muscle cell or a red blood cell. Since stem cells have the ability to turn into various other types of cells, scientists believe that they can be useful for treating and understanding diseases. Thus, stem cells have widened horizon of scope of research potential and therapies too.

Recently , Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and tissue engineering due to their evident advantages in providing more physiologically relevant information and more predictive data for in vivo tests. The innovations and development in 3D culture systems for drug discovery over the past 5 years are also reviewed in the article, emphasizing the cellular response to different classes of anticancer drugs, focusing particularly on similarities and differences between 3D and 2D models across the field. The progression and advancement in the application of 3D cell cultures in cell-based biosensors .

3D cell culture is an in-vitro technique wherein the cells can grow in an artificially created environment. These environments closely resemble the architecture and functioning of the native tissue. 3D cell culture technique helps stimulate cell differentiation, proliferation, and migration by interacting with their three-dimensional surroundings as they would in the in-vivo environment. As 3D cell cultures can mimic the structure, activity, and microenvironment of the in-vivo tissues, this technique has varied applications in the fields of drug screening, regenerative medicine, stem cell therapies, cancer research and cell biology. The extracellular matrix in 3D cell cultures enables cell–cell communication by direct contact as in in-vivo environment by secreting cytokines and trophic factors. These factors are changed in a 2D environment that can significantly affect the cell–cell communication, which in turn can alter the cell morphology and proliferation. As 2D cultures cannot recapitulate the architecture and complex cellular matrices as in 3D cultures, this technique is gaining popularity in healthcare research sector. In addition, 3D cell cultures can provide results with improved efficiency and reduce the cost of overall R&D process.

Broadly, 3D cell culture techniques are classified as Scaffold-based or non-scaffold-based techniques.

In scaffold based techniques cells are grown in presence of a support. 2 major types of support can be used:

1. Hydrogel-based support: Hydrogels are by definition polymer networks extensively swollen with water. Cells can be embedded in these hydrogels or simply coated at the surface. Depending on the nature of the polymer, hydrogels can be classified in to different categories (ECM protein-based hydrogels, natural hydrogels and synthetic hydrogels) with distinct properties.

2. Polymeric hard material based support: cells are cultivated in presence of fibers or sponge-like structures: cell recover a more physiological shape because they are not plated on a flat surface. Materials used for these supports can be polystyrene (adapted for imaging studies because of its transparency) but also biodegradable tools like polycaprolactone.

Scaffold-free techniques allow the cells to self-assemble to form non-adherent cell aggregates called spheroids. Spheroids mimic the solid tissues by secreting their own extracellular matrix and displaying differential nutrient availability. Spheroids grown via non-scaffold based techniques are consistent in size and shape and are better in-vitro cellular models for high-throughput screening The evolution of 3D cell culture has the potential to bridge the gap between in-vitro and in vivo experiments. The convenience of handling cells in-vitro while obtaining results that reflect in-vivo condition and avoiding ethical concerns of animal usage is making 3D cell culture techniques increasingly popular among researchers, but choosing the right system to develop a 3D cell culture model is not a trivial question.

FISH

From oncologist perspective, we perform FISH to help in both the diagnosis of genetic disease or suggest prognostic outcome. We detect genetic abnormalities such as characteristic gene fusion, aneuploidy, and loss of a chromosomal region / whole chromosome or to suggest prognostic outcomes.

FACS is an abbreviation for fluorescence-activated cell sorting, which is a flow cytometry technique that further adds a degree of functionality. By utilizing highly specific antibodies labelled with fluorescent conjugates, FACS analysis allows us to simultaneously collect data on, and sort a biological sample by a nearly limitless number of different parameters.

Invitro Fertilisation

IVF – In Vitro Fertilization What is In Vitro Fertilization (IVF)? In Vitro Fertilization is an assisted reproductive technology (ART) commonly referred to as IVF. IVF is the process of fertilization by extracting eggs, retrieving a sperm sample, and then manually combining an egg and sperm in a laboratory dish. The embryo(s) is then transferred to the uterus. Other forms of ART include gamete intrafallopian transfer (GIFT) and zygote intrafallopian transfer (ZIFT).